For a lot more than fifty percent a ten years I have suffered from lactose intolerance. If I eaten even the smallest amounts of lactose I would be violently sick within just the hour. So as I was selecting my college I vowed that one working day I would deal with it.

A short while ago my close friend Gabriel Licina, renowned biohacker and genetic engineer, gave me entry to the equipment and products I required to make a treatment that could potentially deal with the concern. The treatment employs a virus termed an AAV to produce DNA that codes for the enzyme that breaks down lactose. When packaged into a pill and ingested, the virus ought to induce my intestine to generate lactase, correctly restoring lactose tolerance.

This challenge was primarily based on investigate done by other experts and I have bundled hyperlinks to all the papers below. I have also bundled hyperlinks to all of the destinations I bought the viral DNA kits from. The other reagents and media have been all obtained from the thermofisher catalogue.

I’m also operating on a writeup of this technique and will with any luck , publish a paper on the final results as soon as I have collected a lot more details. I know that so far my sample sizing is nonetheless only N=1 but my existence has adjusted from this challenge and I required to share my development. I’m not likely to make any claims about the challenge other than my existence seems to have enhanced.

This is for educational needs only. Do not endeavor just about anything you see, unless you are an skilled biologist.


Unique paper:
pAAVLacz Plasmid:
AAV package:


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